Water-soluble enzyme-polymer grafts: Thermal stabilization of glucose oxidase

Thermal stabilization of glucose oxidase and glucoamylase by physical entrapment.

Physical entrapment was used as an approach to achieve thermal stabilization of enzymes. The t 1/2 values for the thermoinactivation of glucose oxidase and glucoamylase were increased several-fold by their entrapment in polyacrylamide gels. In polyacrylate gels the individual enzymes behaved differently, probably owing to microenvironmental effects arising by the polyelectrolyte nature of the c...

Preparation of a Water-Soluble Fluorescent Polymer

A fluorophore-enriched polymer was synthesized through modification of a kind of copolymer by fluorescein isothiocyanate (FITC). The copolymer was obtained by batch emulsion polymerization of t-butyl acrylate (t-BA) and an amino-containing monomer M2, which was produced in our laboratory. According to various characterizations, such as gel permeability chromatography (GPC), H-NMR, fluorescence ...

Glucose-sensitivity of glucose oxidase-containing cationic copolymer hydrogels having poly(ethylene glycol) grafts.

Glucose oxidase and catalase were immobilized on poly(diethylaminoethyl methacrylate-g-ethylene glycol) gels by copolymerization of the constituent monomers and the functionalized enzyme solutions. The hydrogels were prepared in the form of discs and microparticles. The amount and the activity of enzymes immobilized in the matrix were determined. The hydrogels were tested for their response to ...

Irreversible thermal denaturation of Glucose oxidase from Aspergillus niger

Polymer-surfactant nanoparticles for sustained release of water-soluble drugs.

Poor drug encapsulation efficiency and rapid release of the encapsulated drug limit the use of nanoparticles in biomedical applications involving water-soluble drugs. We have developed a novel polymer-surfactant nanoparticle formulation, using the anionic surfactant Aerosol OT (AOT) and polysaccharide polymer alginate, for sustained release of water-soluble drugs. Particle size of nanoparticles...